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Research on Crops

 Complex biofungicide novoсhizol-S and its components: Cytophysiological assessment of effectiveness for treating bread wheat​



DOI: 10.31830/2348-7542.2025.ROC-1249    | Article Id: ROC-1249 | Page : 562-568
Citation :- Complex biofungicide novoсhizol-S and its components: Cytophysiological assessment of effectiveness for treating bread wheat​. Res. Crop. 26: 562-568
PLOTNIKOVA LYUDMILA YA, KNAUB VALERIYA V, SKOLOTNEVA EKATERINA S, KELBIN VASILIY N, FOMENKO VLADISLAV V AND SHCHERBAN ANDREY B lya.plotnikova@omgau.org
Address : Omsk State Agrarian University named after P.A. StolypinInstitutskaya pl. 1, Omsk, 644008, Russia
Submitted Date : 12-10-2025
Accepted Date : 30-11-2025
Online Published:

Abstract

Chitosan-based preparations are promising for the biological plant protection from fungal diseases. The biopesticide novochizol (Nov) is a globular form of chitosan and has several advantages over linear derivatives. The effect of Nov can be enhanced by creating complexes with substances having a synergistic effect. The aim of the work was to evaluate the effectiveness of the complex Nov with the sulphur (Nov-S) and its separate components for the defense of bread wheat Triticum aestivum L. against stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt). The experiments were carried out at the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of   Sciences, and the cytological studies were performed at Omsk State Agrarian University (Russia) in 2025. The studies were carried out on seedlings of the susceptible to stem rust spring bread wheat variety infected with a sample of the Pgt population. During the experiment, plant reactions to infection were assessed after pre-treatment with Nov, colloidal S, and Nov-S. To understand the effects of the preparations, studies were carried out on the pathogen development and the dynamics of defense reactions using cytochemical methods. On Nov-treated plants, partial suppression of the appressoria development on the leaf surface, and disruption of penetration into the tissues due to Reaction Oxygen Species (ROS) generation on the stomata, and the induction of hydrogen peroxide and phenolic substances synthesis were observed. The fungicidal S affect led to the full inhibition of pathogen development before penetration into the stomata, but significant damage of plant tissues was noted. Pre-treatment with the Nov-S complex caused a sharp decrease in the pustule number per leaf (22 times compared to the control) with minimal plant damage. The main protective mechanisms of Nov-S-treated were the inhibition of appressoria formation and fungus stopping after the penetration into the stomata. There was an intensive accumulation of H2O2 and phenolic substances with different spectral characteristics in the zones of rare colonies and small pustules. The Nov-S complex is of interest as a biofungicide for protecting wheat from rust diseases.

Keywords

Novochizol stem rust Sulfur wheat 

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